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omnilog® pm-m system  (Biolog Inc)

 
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    Biolog Inc omnilog® pm-m system
    Omnilog® Pm M System, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omnilog+pm-m+system/10__3390_slash_genes15111362-172-6-6?v=Biolog+Inc
    Average 90 stars, based on 1 article reviews
    omnilog® pm-m system - by Bioz Stars, 2026-08
    90/100 stars

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    a . Schematic representation of metabolite utilization analysis workflow. Nutrient utilization profile as measured by Biolog (using the Omnilog device) was used to determine metabolite consumption that correlates with gene expression. b. Heatmap showing the differential use of metabolites by 19 PDA cells in nutrient-depleted media over 74.5 h culture. Red denotes over-utilization; blue denotes under-utilization. PM-M1 plate contains mainly carbohydrates. c. Heatmap showing metabolites that were utilized mainly a subset of cell lines. d . Heatmap showing PDA cell lines stratified into the high and low uridine consumers. e . Measurement of relative metabolic activity (RMA) of PDA cell lines cultured in media supplemented with 1 mM uridine for 74.5 h. Basal media indicates SILAC RPMI 1640 supplemented with 5% dFBS, 0.3mM Gln, 1.148mM Arg, and 0.2189 mM Lys.

    Journal: bioRxiv

    Article Title: Nutrient profiling reveals extracellular uridine as a fuel for pancreatic cancer through uridine phosphorylase 1

    doi: 10.1101/2021.06.07.447448

    Figure Lengend Snippet: a . Schematic representation of metabolite utilization analysis workflow. Nutrient utilization profile as measured by Biolog (using the Omnilog device) was used to determine metabolite consumption that correlates with gene expression. b. Heatmap showing the differential use of metabolites by 19 PDA cells in nutrient-depleted media over 74.5 h culture. Red denotes over-utilization; blue denotes under-utilization. PM-M1 plate contains mainly carbohydrates. c. Heatmap showing metabolites that were utilized mainly a subset of cell lines. d . Heatmap showing PDA cell lines stratified into the high and low uridine consumers. e . Measurement of relative metabolic activity (RMA) of PDA cell lines cultured in media supplemented with 1 mM uridine for 74.5 h. Basal media indicates SILAC RPMI 1640 supplemented with 5% dFBS, 0.3mM Gln, 1.148mM Arg, and 0.2189 mM Lys.

    Article Snippet: The reduction of the dye over time was measured as absorbance (A590-A750) using the OmniLog® PM-M instrument (Biolog, 93171) for 74.5 hours at 15 minutes intervals.

    Techniques: Expressing, Activity Assay, Cell Culture